| Autor: |
Fibi, MR, Stuber, W, Hintz-Obertreis, P, Luben, G, Krumwieh, D, Siebold, B, Zettlmeissl, G, Kupper, HA |
| Zdroj: |
Blood; March 1991, Vol. 77 Issue: 6 p1203-1210, 8p |
| Abstrakt: |
Five different peptides (P1: 84'95; P2: 152'166; P3: 52'63; P4: 7'23; P5: 110'123) homologous to relatively hydrophilic regions of human erythropoietin (huEpo) have been synthesized to identify biologically active domains of the hormone. All peptides were able to induce high titers of peptide-specific antibodies in rabbits. Antisera from rabbits induced by recombinant huEpo (rhuEpo) contained a relatively high amount of antibodies preferentially directed against three peptides (P2, P4, and P5), of which P4 comprised the amino-terminal region, P2 the carboxyl-terminus, and P5 an interior region previously described as the receptor-binding site. The same three peptides were able to induce rhuEpo-specific antibodies, whereas P1 and P3 lacked this activity. Only peptide-P2-induced antisera inhibited the biologic activity of rhuEpo in a cell proliferation assay, indicating that the carboxyl-terminal region of the molecule is essentially involved in the biologic function of rhuEpo. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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