| Autor: |
Limentani, SA, Furie, BC, Poiesz, BJ, Montagna, R, Wells, K, Furie, B |
| Zdroj: |
Blood; November 1987, Vol. 70 Issue: 5 p1312-1315, 4p |
| Abstrakt: |
Immunoaffinity chromatography using conformation-specific antibodies yields pure factor IX from human plasma in a single rapid, facile purification step. We evaluated this technique to determine whether factor IX can be separated from human T cell leukemia virus-I (HTLV-I) and human immunodeficiency virus (HIV) in plasma supplemented with these viruses. Viral content was determined with an enzyme-linked immunosorbent (ELISA) assay sensitive to 50 ng viral protein. Both HTLV- I and HIV coeluted with unbound protein. Neither HTLV-I nor HIV was detected in purified factor IX. We conclude that, to the limits of detection, factor IX purified by this method is free of viral contamination. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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