| Abstrakt: |
Convolvulus pluricaulisis a highly valued rasayana drug of the Ayurvedic system of medicine. It is one of the four drugs (Convolvulus pluricaulis, Evolvulus alsinoides, Clitoria ternatea, and Canscora decussata) described in Ayurveda as Shankhpushpi. Because C. pluricaulisis described and used as Shankhpushpi by most practitioners, the objective of this study was to develop an HPTLC method for generation of a distinct chemical profile of Shankhpushpi and for quantification of scopoletin in C. pluricaulisand in commercial formulations containing the plant. Because scopoletin is one of the major coumarins in C. pluricaulis, it was used as a chemical marker. Chromatography on silica gel, with toluene–ether, 1 + 1, saturated with 10% glacial acetic acid, as mobile phase enabled good resolution of scopoletin without interference from other compounds present in Shankhpushpi. Scopoletin was resolved at RF0.31 as a blue fluorescent spot when scanned at λ = 366 nm. The study was extended to the analysis of commercial samples and formulations containing Shankhpushpi. Scopoletin was found to be present in almost all the commercial samples and formulations. Three other coumarins of RF0.47, 0.55, and 0.63 were present in some of the samples. The HPTLC results were confirmed by results from UV spectroscopic scanning of the samples at λ = 366 nm. The method was validated for linearity (400–1200 ng/spot), correlation coefficient (0.9881), accuracy (recovery = 98.3%), precision (or CV, %; intraday 1.5–3.3%, interday 3.5–5.5%), limit of detection (50 ng/spot), and limit of quantification (100 ng/spot). The method is simple, sensitive, and precise and can be used for routine quality-control testing of C. pluricaulisand its commercial formulations. |
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