| Autor: |
Tsai, Jo C., Liu, Lixin, Cooley, Brian C., DiChiara, Maria R., Topper, James N., Aird, William C. |
| Zdroj: |
The FASEB Journal; October 2000, Vol. 14 Issue: 13 p1870-1872, 3p |
| Abstrakt: |
Egr‐1 is an immediate early gene that couples short‐term changes in the extracellular milieu to long‐term changes in gene expression. Under in vitroconditions, the Egr‐1 gene is expressed in many cell types and is induced by a wide variety of extracellular signals. The mechanisms by which the Egr‐1 gene is regulated in vivoremain poorly understood. In this study, we have generated transgenic mice with a construct containing 1200 bp of the mouse Egr‐1 promoter coupled to nuclear localized LacZ. In multiple independent lines of mice, reporter gene expression was detected in subsets of endothelial cells, vascular smooth‐muscle cells, cardiomyocytes, neurons, and hepatocytes. This pattern closely resembled that of the endogenous gene. After partial hepatectomy, reporter gene activity was upregulated between two‐ and fivefold in regenerating livers. Taken together, these findings suggest that the Egr‐1 promoter contains information for appropriate spatial and temporal expression in vivo. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Plný text