| Autor: |
Bakar, Nur Husnina Iffah, Lee, Thong Chuan, Mohd-Hairul, Ab. Rahim, Raus, Raha Ahmad, Basri, Dayang Fredalina, Adzahar, Noor Suhana |
| Zdroj: |
Materials Science Forum; March 2020, Vol. 981 Issue: 1 p265-270, 6p |
| Abstrakt: |
A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. |
| Databáze: |
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