| Autor: |
Goh, Shu Ling, Kee, Boon Pin, Abdul Jabar, Kartini, Chua, Kek Heng, Nathan, Anna Marie, Bruyne, Jessie, Ngoi, Soo Tein, Teh, Cindy Shuan Ju |
| Zdroj: |
Pathogens and Global Health; January 2020, Vol. 114 Issue: 1 p46-54, 9p |
| Abstrakt: |
ABSTRACTStreptococcus pneumoniae(S. pneumoniae) is one of the main causative agents of pneumococcal diseases. To date, more than 90 distinct serotypes have been identified. Implementation of vaccines has caused a drastic reduction in vaccine-serotype pneumococcal diseases but increase in cases due to non-vaccine serotype has been observed in Malaysia. However, further investigation on different serotype incidence in Malaysia is needed and the rate of pneumococcal vaccination for new-born babies in Malaysia remains low. The recent emergence of drug-resistant S. pneumoniae(DRSP) has also been a global concern, especially penicillin resistance. This study determined the serotypes of S. pneumoniaestrains (n= 95) isolated from nasopharyngeal specimens from children admitted to UMMC from 2013 to 2015. In accordance with previous studies, PCR result showed 40% of NT isolates were successfully typed as 3 less common serotypes, namely 9N/L, 17A, and 23B. The repetitive-element PCR (REP-PCR) result revealed genetic variations among the strains whereby five major clusters were observed at the similarity of 80% by clustering analysis based on fingerprint data. Penicillin-binding proteins (pbps) of selected isolates were studied by PCR and sequencing. Three strains with ≤19-mm diameter zone for Oxacillin Disc Diffusion (ODD) test previously were recorded to have mutation on all pbp1a, pbp2b,and pbp2xwith MIC of 4 µg/ml, which were penicillin-intermediate resistance according to the CLSI breakpoints. |
| Databáze: |
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