| Autor: |
Lee, Jeong Ho, Reddy, Dheerendra K., Saran, Rajiv, Moore, Harold L., Twardowski, Zbylut J., Nolph, Karl D., Khanna, Ramesh |
| Zdroj: |
Peritoneal Dialysis International; December 2000, Vol. 20 Issue: Supplement 5 p39-47, 9p |
| Abstrakt: |
Objective To evaluate and compare the effects of glucose-based solutions to those of icodextrin with respect to peritoneal transport characteristics and formation of advanced glycosylation end-products (AGEs) in the peritoneal membrane in the diabetic rat model of peritoneal dialysis (PD).Study Design Thirty-three male Sprague–Dawley rats weighing between 275 – 300 g were divided into 5 groups: group C (n= 6), control rats with catheter but not dialyzed; group D (n= 5), diabetic rats with catheter but not dialyzed; group G (n= 7), diabetic rats dialyzed with standard 2.5% glucose solution for daytime exchanges and 4.25% glucose solution for the overnight exchange; group H (n= 8), diabetic rats dialyzed with standard 2.5% glucose solution for daytime exchanges and 7.5% icodextrin solution for overnight exchanges; group I (n= 7), diabetic rats dialyzed with 7.5% icodextrin solution for all exchanges. Dialysis exchanges were performed three times daily with an instillation volume of 25 mL per exchange for a period of 12 weeks. Tissue sections were stained using a monoclonal anti-AGE antibody. One-hour peritoneal equilibration tests (PET) were performed every 4 weeks for comparison of transport characteristics.Results The level of immunostaining was lowest in group C and highest in group G. Significant differences were seen between group C and groups G, H, and I (p< 0.001, p= 0.001, and p< 0.05 respectively). Significant differences were also found between group G and groups D and I (p< 0.05 and p< 0.05 respectively). Over time, glucose concentration at the end of an exchange versus concentration at instillation (D/D0glucose) decreased and dialysate-to-plasma ratio (D/P) of urea increased. Significant differences were found between groups C and H for D/D0glucose (0.40 ± 0.01 vs 0.35 ± 0.01, p< 0.05); and between groups C and H for D/P urea (0.87 ± 0.03 vs 0.97 ± 0.02, p< 0.05).Conclusions These results suggest that AGE formation is lower with the use of peritoneal dialysis solution containing icodextrin than with glucose-based solutions. We conclude that the use of icodextrin may be helpful in slowing the deterioration of the peritoneal membrane, prolonging its use for dialysis. |
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