| Autor: |
Abbasi, Shayan, Farahani, Homa, Lanjanian, Hossein, Taheri, Mohammad, Firoozpour, Loghman, Davoodi, Jamshid, Pirkalkhoran, Sama, Riazi, GholamHossein, Pooyan, Shahriar |
| Zdroj: |
Bioconjugate Chemistry; March 2020, Vol. 31 Issue: 3 p708-720, 13p |
| Abstrakt: |
The attachment of PEG to biopharmaceuticals has been applied for enhancement of bioavailability and improved stability. The PEG polymer is highly hydrated; thus effective attachment to inaccessible sites could be hindered. We have devised a scheme to address this issue by introducing a considerable distance between PEG and protein by addition of a linear peptide, appended to long chained reactive linkers. Second, the position of PEG conjugation directly affects biological activity. Accordingly, a disulfide bond could be considered as an ideal choice for site directed PEGylation; but reactivity of both thiol moieties to bridging reagent is critical for maintenance of protein structure. In our design, a forked structure with two arms provides essential flexibility to account for dissociation of reduced cysteines. An efficient yield for disulfide PEGylation of IFN-β1b was attained and specificity, biophysical characterization, biological activity, and pharmacokinetics were surveyed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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