Development and evaluation of on-line nanoliter flow analysis of protein digests by pneumatic-splitter electrospray liquid chromatography mass spectrometry

Autor: Le, John C., Hui, John, Haniu, Mitsuru, Katta, Viswanatham, Rohde, Michael F.
Zdroj: Journal of the American Society for Mass Spectrometry; July 1997, Vol. 8 Issue: 7 p703-712, 10p
Abstrakt: We have developed a novel approach to characterize protein digests by pneumatic-splitter electrospray ionization liquid chromatography mass spectrometry (PSESI-LCMS). This technique involves an interfacing of a pneumatic splitter that can dynamically generate and control a steady split flow rate of nanoliters per minute. An average PSESI-LCMS analysis, depending on the desired split ratio, consumes less than 200 femtomoles of sample. A tryptic digest map of a glycoprotein, fetuin, and a Lys-C digest map of a recombinant neurotrophin protein, neurotrophin-3, are reported here for this investigation.
Databáze: Supplemental Index
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