| Abstrakt: |
Genetic and physiological characteristics of the main rice (Oryza sativaL.) storage proteins, glutelin and prolamin, have been extensively studied. In contrast, information on high molecular weight proteins is limited. Experiments were undertaken to identify the genes that control the synthesis of high molecular weight proteins (40–90 kDa) in rice endosperm. Total endosperm proteins of japonicaand indicacultivars, and their F2progeny, were analyzed by two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE). Nine high molecular weight proteins were detected in three cross combinations between japonicaand indicacultivars. Segregation of these endosperm proteins in the F2progeny fits with a ratio of 1:1:1:1 (HpxHpxHpx:HpxHpxhpx:Hpxhpxhpx:hpxhpxhpx), resulting from double fertilization. Three protein subunits (D, E, and M) were found only in the cross combination of Taichung 65 × BlueBelle, but other six protein subunits (F, G, H, I, K, and L) were commonly in three cross combinations. The dominant genes appeared to regulate expression of the high molecular weight protein subunits, anda dose effect of dominangt enes was detected. |
Plný text