| Autor: |
Shima, Kenji, Hirota, Meisei, Ohboshi, Chizuko, Sato, Mitsuyo, Nishino, Tomoyoshi |
| Zdroj: |
European Journal of Endocrinology; April 1987, Vol. 114 Issue: 4 p531-536, 6p |
| Abstrakt: |
Abstract. A reliable radioimmunoassay for glucagon-like peptide 1 (GLP-1) was developed with a detection limit of 12 pmol/l, which enabled us to detect the subtle change in concentrations of this peptide in the perfusate from the perfused rat pancreas. With this RIA, GLP-1 immunoreactivity (GLP-1 IR) was found to be secreted synchronously with glucagon immunoreactivity upon arginine stimulation from the perfused rat pancreas. Gel chromatographic analysis showed the presence of GLP-1 IR in the pancreatic extract which is eluted at the same position as synthetic GLP-1 (1-37). Moreover HPLC analysis confirmed the presence of GLP-1 IR, which eluted at exactly the same position as synthetic GLP-1, in the perfusate from pancreas perfusion upon stimulation of arginine. These results suggest that the pancreas stores and secretes GLP-1 IR concomitantly with glucagon as one of the cleavage products of their common precursor. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
