| Autor: |
BOUTERS, R., ESNAULT, C., SALISBURY, G. W., ORTAVANT, R. |
| Zdroj: |
Reproduction; December 1967, Vol. 14 Issue: 3 p355-363, 9p |
| Abstrakt: |
The dnacontent of individual ejaculated spermatozoa and of spermatozoa recovered from the epididymis and ampulla of fifteen rabbits was measured by means of two microspectrophotometric methods: (i) direct determination of ultra-violet (uv) light absorbed at 260 mμ by unstained spermatozoa, and (ii) determination of visible light absorbed at 560 mμ by Feulgen-stained spermatozoa. The dnadeterminations in uvlight for ejaculated spermatozoa and for those recovered from the male ducts revealed no difference, as contrasted with the results obtained by measuring the Feulgen-stainability. The differences in Feulgen-stainability, i.e. in the quantitative response of spermatozoa to the Feulgen reagent, are attributed to an ageing process in the sperm cells: ampullary spermatozoa yielded significantly lower Feulgen-dnavalues than those obtained from the epididymis; ligation of the vas deferens resulted in a decrease of the Feulgen-dnacontent of epididymal spermatozoa; the variable Feulgen-dnacontent of individual ejaculated spermatozoa is attributed to varying percentages of older sperm cells in the ejaculates. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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