| Abstrakt: |
The mammalian tachykinin peptides are a family of neuropeptides characterized by a common C‐terminal amino acid sequence of the form Phe‐X‐Gly‐Leu‐Met‐NH2, where X is either Phe or Val. Tachykinin peptides have various biological activities, including excitation of both peripheral and central neurons, stimulation of smooth muscle contraction, stimulation of exocrine and endocrine gland secretion, and involvement in immune and inflammatory responses. This unit presents basic methods for the characterization of tachykinin receptors by radioligand binding assay. The protocols detail methods for three major classes of tachykinin receptors and techniques for the generation of high specific activity iodinated radioligands. |
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