| Abstrakt: |
Brown plant hopper (BPH), Nilaparvata lugens(Stal.), is a serious insect pest of rice in Asia, causing direct losses and vectoring Rice grassy stunt virus(RGSV) and Rice ragged stunt virus (RRSV). Recombinant inbred lines (RILs) developed from a cross between ‘IR50’ and ‘IR54745‐2‐21‐12‐17‐6’ were used to identify random amplified polymorphic DNA (RAPD) markers closely linked to a BPH Biotype‐4 resistance gene [Bph13 (t)] derived from Oryza officinalisWall. Bulked segregant analysis (BSA) using RAPD primers identified 11 polymorphic fragments. Six fragments, AJ09260a, AL05220a, AK10690a, AK10430c, AK10380d, and AJ01200a, were linked in coupling phase to the Bph13 (t)locus. The remaining five fragments, AJ09230b, AJ09180c, AJ09100d, AL05400b, and AK10340e, were linked in repulsion. The most closely linked RAPD marker, AJ09230b, was converted to a codominant linked sequence tagged sites (STS) marker. This marker mapped 1.3 centimorgans (cM) from the resistance gene and was placed on rice chromosome 3 by means of ‘IR64’ × ‘Azucena’ doubled haploid (DH) population. The tightly linked STS marker could be used for marker‐assisted selection (MAS). In addition, these markers will be useful for a positional cloning strategy to isolate the resistance gene. |
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