Next‐Generation Sequencing Fragment Library Construction

Autor:	Podnar, Jessica, Deiderick, Heather, Hunicke‐Smith, Scott
Zdroj:	Current protocols in molecular biology; July 2014, Vol. 107 Issue: 1 p7.17.1-7.17.16
Abstrakt:	All current next‐generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5′ and 3′ flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results. Curr. Protoc. Mol. Biol. 107:7.17.1‐7.17.16. © 2014 by John Wiley & Sons, Inc.
Databáze:	Supplemental Index
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