| Autor: |
Richter, Monique, Negro‐Demontel, María Luciana, Blanco‐Ocampo, Daniela, Taranto, Eliseo, Lago, Natalia, Peluffo, Hugo |
| Zdroj: |
Current Protocols In Immunology; January 2018, Vol. 120 Issue: 1 p24.1.1-24.1.25 |
| Abstrakt: |
Traumatic brain injury (TBI) is a leading cause of death and disability and is a risk factor for the later development of neuropsychiatric disorders and neurodegenerative diseases. Many models of TBI have been developed, but their further refinement and a more detailed long‐term follow‐up is needed. We have used the Thy1‐YFP‐H transgenic mouse line and the parallel rod floor test to produce an unbiased and robust method for the evaluation of the multiple effects of a validated model of controlled cortical injury. This approach reveals short‐ and long‐term progressive changes, including compromised biphasic motor function up to 85 days post‐lesion, which correlates with neuronal atrophy, dendrite and spine loss, and long‐term axonal pathology evidenced by axon spheroids and fragmentation. Here we present methods for inducing a controlled cortical injury in the Thy1‐YFP‐H transgenic mouse line and for evaluating the resulting deficits in the parallel rod floor test. This technique constitutes a new, unbiased, and robust method for the evaluation of motor and behavioral alterations after TBI. © 2018 by John Wiley & Sons, Inc. |
| Databáze: |
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