| Autor: |
Zhang, Jing, Eswaran, Gugan, Alonso-Serra, Juan, Kucukoglu, Melis, Xiang, Jiale, Yang, Weibing, Elo, Annakaisa, Nieminen, Kaisa, Damén, Teddy, Joung, Je-Gun, Yun, Jae-Young, Lee, Jung-Hun, Ragni, Laura, Barbier de Reuille, Pierre, Ahnert, Sebastian E., Lee, Ji-Young, Mähönen, Ari Pekka, Helariutta, Ykä |
| Zdroj: |
Nature Plants; October 2019, Vol. 5 Issue: 10 p1033-1042, 10p |
| Abstrakt: |
Vascular cambium, a lateral plant meristem, is a central producer of woody biomass. Although a few transcription factors have been shown to regulate cambial activity1, the phenotypes of the corresponding loss-of-function mutants are relatively modest, highlighting our limited understanding of the underlying transcriptional regulation. Here, we use cambium cell-specific transcript profiling followed by a combination of transcription factor network and genetic analyses to identify 62 new transcription factor genotypes displaying an array of cambial phenotypes. This approach culminated in virtual loss of cambial activity when both WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNOTTED-like from Arabidopsis thaliana1 (KNAT1; also known as BREVIPEDICELLUS) were mutated, thereby unlocking the genetic redundancy in the regulation of cambium development. We also identified transcription factors with dual functions in cambial cell proliferation and xylem differentiation, including WOX4, SHORT VEGETATIVE PHASE (SVP) and PETAL LOSS (PTL). Using the transcription factor network information, we combined overexpression of the cambial activator WOX4and removal of the putative inhibitor PTLto engineer Arabidopsisfor enhanced radial growth. This line also showed ectopic cambial activity, thus further highlighting the central roles of WOX4and PTLin cambium development. |
| Databáze: |
Supplemental Index |
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