| Autor: |
McAvin, James C, McConathy, Marlana A, Rohrer, Andrew J, Huff, William B, Barnes, William J, Lohman, Kenton L |
| Zdroj: |
Military Medicine; October 2003, Vol. 168 Issue: 10 p852-855, 4p |
| Abstrakt: |
Real-time fluorescence polymerase chain reaction is a microbial identification method that can provide rapid and accurate results using a field-deployable thermocycler, the RAPID ("ruggedized" advanced pathogen identification device). A Yersinia pestis-specific TaqMan assay required approximately 75 minutes and achieved a sensitivity of 100 fg of Y. pestis genomic DNA (20 genome equivalents). Specificity testing against a genomic DNA cross-reaction panel comprised of 22 bacterial species encountered in the respiratory tract resulted in no false positives. No cross-reaction occurred with human genomic DNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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