| Autor: |
Wachsman, M., Aurelian, L., Hunter, J. C. R., Perkus, M. E., Paoletti, E. |
| Zdroj: |
Bioscience Reports; August 1988, Vol. 8 Issue: 4 p323-334, 12p |
| Abstrakt: |
We studied the effect of the temporal regulation of herpes simplex virus (HSV) type 1 glycoprotein D (gD-1) expression in Ia+ epidermal cells (EC) and macrophages on virus specific immunity and protection from HSV-2 challenge. gD-1 was expressed on the surface of cells infected with a vaccinia recombinant containing gD-1 under the control of an early vaccinia virus promoter (VP176). It was not expressed in cells infected with a recombinant (VP254) in which gD-1 is controlled by a late vaccinia virus promoter. BALB/c mice immunized with both recombinants seroconverted to HSV-2 as determined by neutralization. However, HSV specific delayed type hypersensitivity (DTH) responses were significantly (p<0.025) higher in VP176 than VP254 immunized animals. Both VP176 and VP254 immunized mice were protected from severe neurological disease due to HSV-2 challenge at 14 days post immunization, but long term protection was observed only in VP176 immunized mice. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink