| Autor: |
Moraru, I. I., Manciulea, Mioara, Cǎlugǎru, Anca, Ghyka, Gr., Popescu, L. M. |
| Zdroj: |
Bioscience Reports; September 1987, Vol. 7 Issue: 9 p731-736, 6p |
| Abstrakt: |
A novel approach was used to assess the role of phosphoinositide hydrolysis in the mitogenic action of phytohemagglutinin (PHA) or concanavalin A (ConA). The treatment of human peripheral blood leukocytes (PBL) with monospecific antibodies against phospholipase C (PLC) produced a dose-dependent inhibition (up to 100%) of PHA (10 μg/ml) or ConA (25 μg/ml) proliferative effects. Thus, the activation of membrane-bound PLC is a sine-qua-non condition for lectin-induced proliferation of T lymphocytes. The key-role of PLC versus protein kinase C (PKC) is stressed by the fact that the inhibition of PKC with Hidaka's compound H-7 (40 μM) produced only a partial blockade (about 25%) of lectin mitogenic effect. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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