| Autor: |
Benzakour, O, Formstone, C, Rahman, S, Kanthou, C, Dennehy, U, Scully, M F, Kakkar, V V, Cooper, D N |
| Zdroj: |
Biochemical Journal; June 1995, Vol. 308 Issue: 2 p481-485, 5p |
| Abstrakt: |
The presence of specific binding sites for the coagulation factor protein S (PS) on the surface of human vascular smooth muscle cells (HVSMC) is described. The binding characteristics of 125I-PS to HVSMC were studied and found to be saturable, reversible and, as described by the Hill equation, co-operative (h 1.74; Kd 0.33 nM). Autoradiographic analysis of detergent extracts of HVSMC chemically cross-linked with 125I-PS and separated by SDS/PAGE revealed radioactivity associated with two proteins with apparent molecular masses of 220 and 230 kDa respectively. The mitogenic activity of PS on HVSMC was also investigated. Protein S was shown to stimulate DNA synthesis of growth-arrested HVSMC and to support their proliferation under low-serum conditions in a sustained and dose-dependent manner. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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