| Autor: |
Gitlin, G, Bayer, E A, Wilchek, M |
| Zdroj: |
Biochemical Journal; March 1987, Vol. 242 Issue: 3 p923-926, 4p |
| Abstrakt: |
Egg-white avidin was treated with 1-fluoro-2,4-dinitrobenzene. Modification of an average of one lysine residue per avidin subunit caused the complete loss of biotin binding. Tryptic peptides obtained from the 2,4-dinitrophenylated avidin were fractionated by reversed-phase h.p.l.c. Three peptides contained the 2,4-dinitrophenyl group. Amino acid analysis revealed that lysine residues 45, 94 and 111 are modified and probably comprise part of the biotin-binding site. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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