| Autor: |
Turnbull, D M, Sherratt, H S A, Davies, D M, Sykes, A G |
| Zdroj: |
Biochemical Journal; September 1982, Vol. 206 Issue: 3 p511-516, 6p |
| Abstrakt: |
A recently described direct reading assay for beta-oxidation and for succinate oxidation in intact mitochondria using [Fe(CN)6]3- as final electron acceptor [Osmundsen & Bremer (1977) Biochem. J. 164. 621-633] has been improved by using instead tetracyano-2,2-bipyridineiron(III) [Fe(CN)4(bpy)]-, which gives a 2.6 times greater absorbance change on reduction. Some physical and kinetic properties of [Fe(CN)4(bpy)]- are described. The use of exogenous cytochrome c(III) as electron acceptor was also tested; this gives the largest absorbance change, although the absolute rate of reaction is only approx. one-third of that using [Fe(CN)6]3- or [Fe(CN)4(bpy)]-. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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