| Autor: |
Bower, D J, Errington, L H, Wainwright, N R, Sime, C, Morris, S, Clayton, R M |
| Zdroj: |
Biochemical Journal; February 1982, Vol. 201 Issue: 2 p339-344, 6p |
| Abstrakt: |
Double-stranded complementary DNA (cDNA) sequences were prepared from day-old chick lens total polysomal RNA and inserted into the unique PstI restriction site of the plasmid pBR322. Colonies containing sequences complementary to abundant lens poly(A)-containing RNA sequences were identified by using lens 32P-labelled cDNA. Some of these clones have been characterized as containing delta-crystallin mRNA coding sequences by genomic DNA blot hybridization and RNA blot hybridizations. Hybridization of labelled DNA from such clones to RNA blots detected four size classes of delta-crystallin RNA sequences, although Southern blots indicated that there are probably only two delta-crystallin genes. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
