Degradation of [3H]chondroitin 4-sulphate and re-utilization of the [3H]hexosamine component by the isolated perfused rat liver

Autor: MacNicholl, A D, Wusteman, F S, Winterburn, P J, Powell, G M, Curtis, C G
Zdroj: Biochemical Journal; January 1980, Vol. 186 Issue: 1 p279-286, 8p
Abstrakt: Radiolabelled chondroitin 4-sulphate was isolated after incubation of rat rib cartilage with N-acetyl-D-[6-3H]galactosamine. After proteolytic digestion of the tissue with either papain or trypsin the released [3H]chondroitin 4-sulphate was added to an isolated perfused rat liver system. Analysis of perfusate after several hours perfusion showed that radiolabelled amino sugars were secreted by the liver in a low-molecular-weight form and as components of glycoproteins.
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