Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures

Autor: Lichtner, R, Wolf, H U
Zdroj: Biochemical Journal; September 1979, Vol. 181 Issue: 3 p759-761, 3p
Abstrakt: A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2–4 degrees C and pH values of 2–4 and an appropriate buffer system containing Li+ or Tris [CH2OH–C(CH2OH)2–NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.
Databáze: Supplemental Index