A sensitive radiochemical assay for angiotensin-converting enzyme (kinase II)

Autor: Chiu, A T, Ryan, J W, Ryan, U S, Dorer, F. E.
Zdroj: Biochemical Journal; July 1975, Vol. 149 Issue: 1 p297-300, 4p
Abstrakt: [[125I]Tyr8]Bradykinin is degraded by angiotensin-converting enzyme to [125I]Tyr-Arg. The reaction product can be separated completely and recovered nearly quantitatively from unchanged substrate by cation-exchange chromatography. Thus it is possible to use [[125I]Tyr8]bradykinin at high specific radioactivity (about 400Ci/mmol) to measure the small quantities of angiotensin-converting enzyme encountered in small-scale cultures of pulmonary endothelial cells.
Databáze: Supplemental Index