Autor: |
Chiu, A T, Ryan, J W, Ryan, U S, Dorer, F. E. |
Zdroj: |
Biochemical Journal; July 1975, Vol. 149 Issue: 1 p297-300, 4p |
Abstrakt: |
[[125I]Tyr8]Bradykinin is degraded by angiotensin-converting enzyme to [125I]Tyr-Arg. The reaction product can be separated completely and recovered nearly quantitatively from unchanged substrate by cation-exchange chromatography. Thus it is possible to use [[125I]Tyr8]bradykinin at high specific radioactivity (about 400Ci/mmol) to measure the small quantities of angiotensin-converting enzyme encountered in small-scale cultures of pulmonary endothelial cells. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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