A simple method of characterizing mitochondrial ribonucleic acid.
| Autor: | Haldar, D, Waters, B K, Freeman, D B |
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| Zdroj: | Biochemical Journal; June 1975, Vol. 147 Issue: 3 p621-623, 3p |
| Abstrakt: | A simple method of isolating and characterizing RNA from L-cell mitochondria is described. The mitochondrial fraction is lysed by sodium dodecyl sulphate, and the RNA fractionated by sucrose-density-gradient centrifugation. The efficacy of proteinase K in preventing ribonuclease activity is also demonstrated. |
| Databáze: | Supplemental Index |
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