| Autor: |
Swanson, Arnold A., Nichols, John T. |
| Zdroj: |
Biochemical Journal; November 1971, Vol. 125 Issue: 2 p575-584, 10p |
| Abstrakt: |
A proteolytic enzyme was isolated from human senile cataractous lens by anion-exchange and gel-filtration chromatography. Sedimentation and zone-electrophoretic experiments indicated a high degree of homogeneity for the enzyme. A molecular weight of 27000 was calculated from measurements of sedimentation velocity and diffusion coefficient. Chelating agents decreased activity which could be restored by addition of certain bivalent metal ions. Di-isopropyl phosphorofluoridate and phenylmethanesulphonyl fluoride inhibit the proteolytic activities. Optimum rates of hydrolysis were observed at pH5.2. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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