| Autor: |
Silva, N.F., Stone, S.L., Christie, L.N., Sulaman, W., Nazarian, K.A.P., Burnett, L.A., Arnoldo, M.A., Rothstein, S.J., Goring, D.R. |
| Zdroj: |
Molecular Genetics and Genomics; May 2001, Vol. 265 Issue: 3 p552-559, 8p |
| Abstrakt: |
Expression of anSreceptor kinase (SRK910) transgene in the self-compatibleBrassica napus cv. Westar conferred on the transgenic pistil the ability to reject pollen from the self-incompatibleBrassica napusW1 line, which carries the S910allele. In one of theSRKtransgenic lines, 1C, virtually no seeds were produced when the transgenic pistils were pollinated with W1 pollen (Mean number of seeds per pod = 1.22). This response was specific to the W1 pollen since pollen from a different self-incompatibleBrassica napusline (T2) and self-pollinations were fully compatible. Westar plants expressing anSlocus glycoprotein transgene (SLG910) did not show any self-incompatibility response towards W1 pollen. Transgenic Westar plants resulting from crosses between the 1CSRKtransgenic line and threeSLG910transgenic lines were also tested for rejection of W1 pollen. The additional expression of theSLG910transgene in theSRK910transgenic plants did not cause any significant further reduction in seed production (Mean seeds/pod = 1.04) or have any detectable effects on the number of pollen grains that adhered to the pistil. Thus, while the allele-specificSLGgene was previously reported to have an enhancing effect on the self-incompatibility response, no evidence for such a role was found in this study. |
| Databáze: |
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