| Autor: |
Hecht, Leonie-Sophie, Jurado-Jimenez, Angeles, Hess, Markus, Halas, Hussein El, Bochenek, Gregor, Mohammed, Hala, Alzahrani, Fahd, Asiri, Mohammed O, Hasan, Rami, Alamri, Aref, Alotaibi, Sultan |
| Zdroj: |
Future Microbiology; July 2020, Vol. 14 Issue: 11 p941-948, 8p |
| Abstrakt: |
Aim:We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. Material & methods:33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1aand upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. Results:18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. Conclusion:The results suggest, that the RealStar®MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO. |
| Databáze: |
Supplemental Index |
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