| Autor: |
Ladeira, M. M., Teixeira, P. D., Gionbelli, M. P., Chizzotti, M. L., Carvalho, J. R. R., Oliveira, D. M., Coelho, T. C. |
| Zdroj: |
Journal of Animal Science; October 2016, Vol. 94 Issue: 1, Number 1 Supplement 5 p422-423, 2p |
| Abstrakt: |
The objective was to evaluate expression of genes involved in lipid metabolism and chemical composition of longissimus dorsi(LD) muscle of Nellore and Angus bulls fed whole shelled corn (WSC) or a ground corn (GC) diet. Twenty-eight bulls with average initial body weight of 378 ± 8.7 kg were used in a completely randomized design and arranged as a 2 × 2 factorial (2 breeds and 2 diets). The GC diet had 30% corn silage and 70% of a concentrate based on corn and soybean meal. The WSC diet had 85% whole shelled corn and 15% of a pellet based on soybean meal and minerals. After being harvested, samples were taken from the LD muscle between the 12th and 13th ribs for centesimal composition analyses and gene expression, which was analyzed by RT-qPCR. The model included the fixed effects of breed, diet, and their interaction. Expression of PPARAwas greater in the LD of Nellore bulls (Table 1; P< 0.01) and also when bulls were fed the WSC diet (P= 0.04). Opposite results were found for SREBF1expression, which was less when bulls were fed the WSC diet (P< 0.01) and less in Nellore bulls (P= 0.03). PPARGand carnitine palmitoyl transferase 2 (CPT2) expression was downregulated in the LD muscle of Nellore bulls fed WSC and upregulated in the LD of Angus fed the same diet. Expression of lipoprotein lipase (LPL), fatty acid binding protein 4 (FABP4), acetyl CoA carboxylase (ACACA), and stearoyl-CoA desaturase (SCD1) was greater (P< 0.05) in the LD of Nellore bulls fed the GC diet. However, diets did not affect the expression of these genes in the LD muscle of Angus bulls. Fatty acid synthase (FASN) expression was greater in the LD of Nellore bulls (P< 0.01) and when animals were fed WSC (P< 0.01). Expression of acyl-coenzyme A oxidase 1 (ACOX) was greater for Angus fed WSC (P= 0.04). Meat from Angus bulls had greater intramuscular fat than meat from Nellore bulls (4.95 and 4.30; P= 0.05). However, there was no effect (P> 0.05) of diet on intramuscular fat. Moisture and protein were not affected (P> 0.05) by diet and breed. In conclusion, expression of PPARAand SREBF1have opposite regulation mechanisms in bovine muscle, regardless of the subspecies. Diets affected the expression of some genes involved in lipid metabolism differently in Nellore and Angus bulls.Table 0878. |
| Databáze: |
Supplemental Index |
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