| Autor: |
Hazell, B.W., Te’O, V.S.J., Bradner, J.R., Bergquist, P.L., Nevalainen, K.M.H. |
| Zdroj: |
Letters in Applied Microbiology; April 2000, Vol. 30 Issue: 4 p282-286, 5p |
| Abstrakt: |
Intact conidia of three industrially relevant strains of Trichoderma reeseiwere effectively transformed by particle bombardment. Transformations were carried out individually with plasmids carrying either the fungal amdSor bacterial hphgene as a selectable marker and by cotransformation with both plasmids. Transformant yields with single plasmids were up to 11 stable transformants per µg DNA at the bombardment distance of 6 cm. Mitotic stability of the transformants was 75–100% and the cotransformation efficiency averaged 92% when the first selection was performed on hygromycin B plates. The entire procedure could be completed in 1 week with the hphmarker. |
| Databáze: |
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