| Autor: |
Kerovuo, J., Tynkkynen, S. |
| Zdroj: |
Letters in Applied Microbiology; April 2000, Vol. 30 Issue: 4 p325-329, 5p |
| Abstrakt: |
Phytase enzymes can increase the nutritional value of food and feed by liberating inorganic phosphate from phytate, the major storage form of phosphorus in plants. The phytase (phyC) from Bacillus subtilisVTT E‐68013 was expressed in Lactobacillus plantarumstrain 755 using Lact. amylovorusα‐amylase secretion signals. In an overnight cultivation in MRS medium containing cellobiose for induction of the α‐amylase promoter, catalytically active phytase was secreted as a predominant extracellular protein. However, Western blot analysis revealed unprocessed and processed phytase in the cell fraction. Pulse chase experiments showed that the recombinant phytase was secreted at a slower rate in comparison to the native proteins of Lact. plantarum755. |
| Databáze: |
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