Development of an acute and chronic ecotoxicity assay using lux‐marked Rhizobium leguminosarumbiovar trifolii

Autor: Paton, G. I., Palmer, G., Burton, M., Rattray, E. A. S., McGrath, S. P., Glover, L. A., Killham, K.
Zdroj: Letters in Applied Microbiology; April 1997, Vol. 24 Issue: 4 p296-300, 5p
Abstrakt: A soil isolate of Rhizobium leguminosarumbv. trifoliiwas marked with a luxCDABE gene cassette to enable the expression of bioluminescence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.
Databáze: Supplemental Index