| Autor: |
Zanotty, Yurisbeth, Álvarez, Marco, Perdomo, Lourdes, Sánchez, Elda E., Giron, María E., Jimenez, Juan C., Suntravat, Montamas, Guerrero, Belsy, Ibarra, Carlos, Montero, Yuyibeth, Medina, Rafael, Navarrete, Luis F., Rodríguez-Acosta, Alexis |
| Zdroj: |
Zebrafish; August 2019, Vol. 16 Issue: 4 p379-387, 9p |
| Abstrakt: |
AbstractEnvenomation by the Venezuelan bushmaster snake (Lachesis muta muta) (Serpentes: Viperidae) is characterized by local and cardiac alterations. This study investigates the in vivocardiac dysfunction, tissue destruction, and cellular processes triggered by Lachesis muta mutasnake crude venom and a C-type lectin (CTL)-like toxin named Mutacytin-1 (MC-1). The 28 kDa MC-1 was obtained by molecular exclusion, ion exchange, and C-18 (checking pureness) reverse-phase chromatographies. N-terminal sequencing of the first eight amino acids (NNCPQ LLM) revealed 100% identity with Mutina (CTL-like) isolated from Lachesis stenophrys, which is a Ca2+-dependent-type galactoside-binding lectin from Bothrops jararacaand CTL BpLec from Bothrops pauloensis.The cardiotoxicity in zebrafish of MC-1 was evaluated by means of specific phenotypic expressions and larvae behavior at 5, 15, 30, 40 and 60 min post-treatment. The L. muta mutavenom and MC-1 also produced heart rate/rhythm alterations, circulation modifications, and the presence of thrombus and apoptotic phenomenon with pericardial damages. Acridine orange (100 μg/mL) was used to visualize apoptosis cellular process in control and treated whole embryos. The cardiotoxic alterations happened in more than 90% of all larvae under the action of L. muta mutavenom and MC-1. The findings have demonstrated the potential cardiotoxicity by L. muta mutavenom, suggesting the possibility of cardiovascular damages to patients after bushmaster envenoming. |
| Databáze: |
Supplemental Index |
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