| Autor: |
Iñiguez, Germán María, Galanti, Marcela, Boric, Maria Angelica, Ferrando, German, Muñoz, Bruno, Beas, Francisco |
| Zdroj: |
Biology of Reproduction; December 1995, Vol. 53 Issue: 6 p1324-1329, 6p |
| Abstrakt: |
Bovine uterotropic placental factor (bUTPF) was obtained from bovine term placentae that had been homogenized and extracted in acidic medium. The extracts were chromatographed on Sephadex G-75, a fraction named native bUTPF was obtained, and an antiserum was generated. The native bUTPF was chromatographed on Sephacryl S-300 HR, and a 230-kDa fraction was obtained. A competitive enzyme immunoassay (EIA) was developed to determine bUTPF levels during gestation. This EIA was linear between 0 and 80 μg/ml (with a detection limit of 0.2 μg/ml) and showed intraassay and interassay coefficients of variation of 11.2% and 15.5%, respectively.Bovine UTPF serum levels during gestation were determined by means of a cross-sectional study between the first month of gestation and delivery. Maximal serum levels of bUTPF were found during the first month of gestation: 7.1 ± 1.9 μg/ml (p< 0.05). In another cross-sectional study, blood samples were collected from 105 cows between 8 and 25 days postinsemination (day of insemination = Day 0). Levels of bUTPF were found to be significantly higher (p< 0.05) by Day 17 compared to Day 0 (5.8 ± 2.2 μg/ml vs. 1.2 ± 0.7 μg/ml). We conclude that bUTPF is detected by EIA in maternal peripheral serum early during gestation in the bovine species. |
| Databáze: |
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