| Autor: |
Wu, Ji, Nayudu, Penelope L., Kiesel, Petra S., Michelmann, Hans W. |
| Zdroj: |
Biology of Reproduction; July 2000, Vol. 63 Issue: 1 p320-327, 8p |
| Abstrakt: |
Although it is known that LH receptors are present from the time of thecal differentiation, the role of LH during early follicle development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in vitro using a culture system that supports the development of intact follicles. We have previously shown that although preantral follicles 150 μm in diameter (2–3 granulosa cell layers) do not require LH to proceed through antral development, smaller follicles (1–2 granulosa cell layers, 85–110 μm in diameter) do not develop beyond the large preantral stage in the presence of only FSH and 5% mouse serum. Follicles of this size were therefore used to determine the effects of LH and serum on their development in vitro. The results showed that although FSH must be continuously present, a low concentration of LH together with a slight increase in serum concentration was necessary, specifically during the primary stage of follicle development (from 85 μm in diameter until the follicles had reached 150 μm in diameter) to induce the capacity for subsequent LH-independent rapid growth and antral development. The in vitro development of maturable oocytes with normal spindle and chromatin morphology was also supported. These results indicate that LH probably induces changes in the early differentiating thecal cells, which are critical for the completion of subsequent follicular and oocyte development. |
| Databáze: |
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