| Autor: |
Donesky, Barry W., de Moura, Marcos Dias, Tedeschi, Cristina, Hurwitz, Arye, Adashi, Eli Y., Payne, Donna W. |
| Zdroj: |
Biology of Reproduction; May 1998, Vol. 58 Issue: 5 p1108-1116, 9p |
| Abstrakt: |
Interleukin (IL)-1β is a putative regulator of ovulation and perhaps luteal function. In this work, we examine its actions on the steroidogenic cascade of the rat granulosa cell. Whereas treatment of immature granulosa cells with FSH for 72 h produced substantial increments in the accumulation of progesterone, the addition of IL-13 produced dose-dependent inhibition of this FSH effect. Pulse labeling of cells with [3H]pregnenolone revealed IL-1 β to effect a decrease in the FSH-supported accumulation of [3H]progesterone while enhancing the accumulation of its proximal metabolite, [3H]20α-dihydroprogesterone. IL-1β was without effect on the activity levels of the progesteronesynthesizing enzymes, even though the corresponding transcripts were elevated. The effect of IL-1 β on some progesteronedegrading enzymes was negligible (5α-reductase) or modest (3α-hydroxysteroid dehydrogenase). In contrast, IL-1 β markedly stimulated both control and FSH-supported 20α-hydroxysteroid dehydrogenase activity (4.8- and 3.3-fold, respectively) and transcripts (16.4- and 7.5-fold, respectively). These data demonstrate an IL-1β-mediated inhibition of gonadotropin-stimulated steroidogenesis via modulation of specific enzymes, and suggest a role for IL-1β in mediating the observed decline of these bioactive hormones during ovulation and luteolysis. |
| Databáze: |
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