| Autor: |
Kenney, Robert M., Evenson, Donald P., Garcia, Marolo C., Love, Charles C. |
| Zdroj: |
Biology of Reproduction; January 1995, Vol. 52 Issue: 1 p647-653, 7p |
| Abstrakt: |
The objectives of this study were to investigate further the sperm chromatin structure assay (SCSA) for use in stallion semen evaluation and to relate sperm chromatin structure to the routine measures of sperm quality as well as to fertility as measured by seasonal pregnancy rate (SPR). Routine clinical fertility and breeding record evaluations and the SCSA were conducted on 106 stallions in commercial breeding operations. Sperm and SCSA values were compared between fertile and subfertile breeding stallions. With the use of the metachromatic DNA probe acridine orange (AO), the SCSA provides a flow cytometric measurement of the relative amounts of double-stranded and single-stranded DNA in each of 5000 sperm cells of the sample population. Under 488-nm laser light, AO that is bound to double-stranded (native) DNA fluoresces green while that bound to single-stranded (denatured) DNA fluoresces red. The ratio alpha t(αt) describes the amount of red to total (red + green) fluorescence emitted. Negative correlations were found between SPR and the SCSA variable COMPαt(cells outside the main population of αt,−0.40), percentage morphologically normal sperm (− 0.41), and percentage progressively motile sperm (− 0.35). The subfertile stallions had higher (p< 0.05) levels of COMPαt(32%) than fertile stallions (16%). The SCSA appears to be useful for estimation of some forms of stallion subfertility. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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