| Autor: |
Padmanabhan, Vasantha, Reno, Kathleen M., Borondy, Maria, Landefeld, Thomas D., Ebling, Francis J.P., Foster, Douglas L., Beitins, Inese Z. |
| Zdroj: |
Biology of Reproduction; May 1992, Vol. 46 Issue: 5 p964-971, 8p |
| Abstrakt: |
Using nutritionally restricted ovariectomized lambs, we tested the hypothesis that nutritionally regulated endogenous in-creases in GnRH secretion (as assessed by LH pulsatility) not only alter the quantity of FSH present in the pituitary and serum, but also alter the pituitary and scrum FSH isoform distribution. Eleven lambs were nutritionally restricted from weaning and ovariectomized at 12 wk of age. Beginning at 56 wk, 6 were fed ad libitum for 14 days, and the other 5 were continued on the restricted diet. Jugular blood samples were collected frequently (12-min interval) for 4 h prior to pituitary removal. Immunoreactive ovine LH (I-oLH) and immunoreactive ovine FSH (I-oFSH) concentrations were measured in sera and pituitary extracts. Bioactive (B) oFSH and I-oFSH Isoform distribution patterns were determined in serum pools and pituitary extracts. Ad libitum feeding increased I-oLH pulsatility and mean concentrations of pituitary and serum I-oFSH and B-oFSH. The I-oFSH isoform distribution patterns in the pituitaries from the nutritionally restricted animals were not different from those of repleted lambs; in both, the predominant FSH peak eluted in the pH range of 3.5–5.6. A similar predominance of I-oFSH isoforms was also evident in the serum of ad libitum-fed animals. This predominance was not demonstrable in 3 of the restricted-fed animals due to low circulating concentrations of FSH ( < 2.5 ng/ml). Subsequent studies, utilizing serum from 4 additional restricted-fed lambs with circulating I-oFSH concentrations in the range of 4–14 ng/ml (but no detectable LH pulses) revealed similar predominance of oFSH isoforms in the pH 3.5–5.6 range. The FSH isoforms eluting in different pH ranges differed in their immunological and biological activities. In summary, our results demonstrate that nutritionally regulated changes in endogenous GnRH secretion do not alter the distribution patterns of FSH isoforms in the pituitary or circulation of restricted- and ad libitum-fed ovariectomized lambs. |
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