| Abstrakt: |
Serum samples were obtained at regular intervals from groups of horses immunized with peripheral blood lymphocytes, thoracic duct lymphocytes, or peripheral blood lymphocyte membranes. These sera were separated into the classical 19 S, 10 S, 7 S, and 4.5 S fractions by Sephadex gel filtration and the antibody activity (antilymphocytic or otherwise) of these fractions, and of the original sera, was assessed by standard in vitro procedures. The antilymphocytic activities measured included lymphocyte agglutination, lysis, and transformation. The other antibodies assayed were platelet and erythrocyte agglutinins and antibodies to a variety of human serum proteins. These studies revealed that (1) there were distinct differences in the rate of appearance of the various antilymphocytic activities studied, the order of appearance being lymphocyte agglutinins, lysins and, finally, transforming antibodies; (2) apart from an initial 19 S agglutinin response (lymphocyte and erythrocytes), all of the activities measured (antilymphocytic or otherwise) were almost exclusively associated with the 7 S fractions of the sera; (3) prolonged immunization was generally associated with a decline in antilymphocytic activities. This may be reversed by resting and/or reboosting the horses; (4) peripheral blood lymphocyte antigens generally gave rise to much higher titres of antibodies to platelets, erythrocytes, and serum proteins than did thoracic duct lymphocytes. The theoretical and practical significance of these results is discussed. |
