| Autor: |
Islam, M.S., Rahim, Z., Alam, M.J., Begum, S., Moniruzzaman, S.M., Umeda, A., Amako, K., Albert, M.J., Sack, R.B., Huq, A., Colwell, R.R. |
| Zdroj: |
Transactions of the Royal Society of Tropical Medicine & Hygiene; January 1999, Vol. 93 Issue: 1 p36-40, 5p |
| Abstrakt: |
It has been hypothesized that Vibrio choleraeis an autochthonous flora of the estuarine and brackish water environment. Zooplankton and phytoplankton have been considered as possible reservoirs. The present study was carried out in microcosms to confirm the role of a cyanobacterium, Anabaenasp., as a reservoir of V. choleraeO1 using culture, polymerase chain reaction (PCR) and immunoelectron microscopy. Survival of culturable V. choleraein microcosms was monitored by using tellurite taurocholate gelatin agar. Culturable V. choleraewere detected for up to 1 h in association with Anabaenasp. from a microcosm. However, viable but nonculturable (VBNC) V. choleraeO1 were detected for up to 25 months using PCR and immunoelectron microscopy. Results also showed that VBNC V. choleraecan multiply and maintain their progeny in the mucilaginous sheath of Anabaenasp. This is the first time that PCR and immunoelectron microscopy have been used to detect nonculturable V. choleraein association with Anabaenasp. This study further clarifies the role of Anabaenasp. as a possible reservoir of cholera. |
| Databáze: |
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