Biochemical and Morphological Changes Following Artificially Stimulated Decidualization in the Mouse Uterus

Autor: Ledford, Barry E., Rankin, Judith C., Markwald, Roger R., Baggett, Billy
Zdroj: Biology of Reproduction; November 1976, Vol. 15 Issue: 4 p529-535, 7p
Abstrakt: A uterine decidual cell reaction was stimulated in hormone primed ovariectomized mice by intraluminal injection of sesame oil. Growth of the stimulated uterine horn occurs in two distinct phases. The first phase begins at approximately 12 h after stimulation and results in a doubling in weight. The second phase begins at approximately 30 h and continues for three to four days resulting in approximately a 20-fold increase in weight. The first growth phase is accompanied by a doubling in protein content and a slight increase in total DNA. The second growth phase is paralleled by increases in both DNA and protein content.Growth of the stimulated uterine horn occurs in discrete masses. Histological examination of stimulated horns reveals the presence of decidual cells 39 h after stimulation. The decidual cell reaction begins at the centers of loculi and spreads centrifugally. Decidualization proceeds grossly and histologically in a pattern similar to that recorded after actual blastocyst contact.
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