| Autor: |
Sacco, Anthony G., Mintz, Beatrice |
| Zdroj: |
Biology of Reproduction; May 1975, Vol. 12 Issue: 4 p498-503, 6p |
| Abstrakt: |
Mouse uterus was analyzed for immunologically specific proteins in the early implantation period (Day 3 of pregnancy in the BALB/c strain). Antiserum was produced in rabbits, absorbed with plasma and other tissues, and subjected to Ouchterlony double diffusion precipitin tests against 16 non-uterine tissues and against uterus from other stages of pregnancy. At least 10–11 soluble antigens were detectable in homogenates of Day 3 pregnant mouse uterus by these methods. Most of the antigens are shared by plasma or are found in non-reproductive tissues, but two uterine antigens are shared only with duodenum. One of the two (designated Band I) may be a uterus-specific product as it is serologically related to, but not identical with, the corresponding duodenal antigen; it shows greatest intensity on Days 3 and 4 of pregnancy. The other (Band II) seems identical in both tissues and decreases as pregnancy progresses. These results are considered in light of our previous observations that the mouse uterus secretes a proteolytic enzyme with peak activity on Day 3 of pregnancy and with an apparent role as an implantation-initiating factor (IIF) causing blastocyst attachment to the uterine wall. The antigens demonstrated in Bands I and II may thus be proteolytic components in uterus and duodenum. The data are consistent with the possibility that the uterus-specific antigen represented by Band I may be the proteolytic IIF and may have a partial relatedness to a proteinase found in the intestine. |
| Databáze: |
Supplemental Index |
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