| Abstrakt: |
Our objectives were to determine the influences of supplemental nonprotein N or protein on feed intake, digestibility, and postabsorptive N metabolism in sheep fed a high-concentrate diet for ad libitum consumption. Nine Romanov-sired, crossbred wethers (13 mo old; 52 kg) were fitted with catheters in a mesenteric artery, mesenteric vein, portal vein, and hepatic vein. Wethers consumed a 95% concentrate diet ad libitum. Treatments consisted of control (no supplemental N; 6.6% CP) or supplemental urea (11.4% CP), soybean meal (SBM; 11.2% CP) or ruminally undegradable protein (BFM; 11.2% CP; 50:50 blood meal and feather meal). Intake or apparently digested intake of DM, OM, and energy did not differ between control and N-supplemented (P> 0.40), or between urea- and protein-supplemented (P> 0.40), but were greater (P< 0.05) in SBM- than in BFM-supplemented wethers. Intake and apparently digested intake of N were less (P< 0.01) in wethers fed the control diet than in those receiving N supplementation but were less (P= 0.03) in BFM- than in SBM-supplemented wethers. Neither portal nor hepatic venous blood flows differed (P> 0.15) among treatments. Net portal release and hepatic uptake of α-amino N and ammonia N and hepatic release of urea N were greater (P< 0.05) in wethers supplemented with N than in controls, but portal-drained viscera (PDV) uptake of urea N did not differ (P> 0.40) among diets. Splanchnic release of a-amino N and ammonia N did not differ from 0 or among diets (P> 0.10), but net release of urea N was less (P= 0.05) for control than for sheep receiving N supplementation. No differences (P> 0.10) in blood concentration within vessel or net flux across PDV, hepatic, or splanchnic tissues of α-amino N, ammonia N, or urea N were observed among wethers receiving supplemental N. Net uptake of oxygen by the PDV did not differ among diets, but hepatic uptake was less (P< 0.05) in control and urea-supplemented sheep than in sheep receiving SBM or BFM. These observations suggest that the source of supplemental N had no large effects on the overall N economy of the animals used in this study. |
