| Autor: |
Schultz, Gilbert A., Harvey, Mark B., Watson, Andrew J., Arcellana-Panlilio, Mayi Y., Jones, Karen, Westhusin, Mark E. |
| Zdroj: |
Journal of Animal Science; January 1996, Vol. 74 Issue: 1, Number 1 Supplement 3 p50-57, 8p |
| Abstrakt: |
A number of growth factor ligand and receptor genes are expressed by early embryos of the mouse, rat, cow, and sheep. Several growth factors have stimulatory effects on early embryos when added exogenously to the medium for culture of preimplantation mammalian embryos. Growth factor supplementation of simple defined medium for culture of early bovine embryos leads to a higher percentage of embryos that can cleave and develop beyond the eightcell stage compared to those in un-supplemented medium. Cultured preimplantation bovine embryos do not express as broad a range of antioxidant enzymes as do mouse embryos. Bovine primary oviduct coculture cells constitutively express the same set of antioxidant enzymes as the mouse embryo and may contribute to protection of the embryo against damage from free oxygen radicals during in vitro culture. Preliminary experiments to analyze growth factor gene expression in cloned bovine embryos indicate that nuclear transplantation alters the normal pattern of expression during early embryonic development. |
| Databáze: |
Supplemental Index |
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