| Autor: |
Pidiyar, Vyankatesh J., Jangid, Kamlesh, Patole, Milind S., Shouche, Yogesh S. |
| Zdroj: |
Mitochondrial DNA: The Journal of DNA Mapping, Sequencing, and Analysis; 2003, Vol. 14 Issue: 3 p183-194, 12p |
| Abstrakt: |
The 16S-23S intergenic spacer and 23S rRNA gene sequence were determined for A. culicicolaMTCC 3249T. Ten different ISR, indicative of ten rrnoperons, were found in the strain that were grouped in three major types. Of the three types, ISR I was non-coding while ISR II and III coded for tRNAGlu(UUC). The tRNAGlu(UUC)sequence was identical to that of E. coli. Both ISR I and II were represented by three clones whereas four clones represented ISR III. The number of nucleotide differences between all these ISR ranged from 4 to 157. There were ten rrnoperons present in A. culicicolaMTCC 3249Tas confirmed by Southern hybridization analysis. The 23S rRNA gene sequence analysis of A. culicicloashowed 89.6% homology to that from E. coliwith differences of 292 bases, whereas it was 98.6% similar to A. hydrophila23S rRNA gene with 38 nucleotide differences. The sequences of the helix 21 region were identical in both A. culicicolaMTCC 3249Tand A. hydrophilaand showed two nucleotides different at 389 and 390th positions as compared to E. coli. The upstream and downstream regions of 23S rRNA gene in the strain showed high sequence similarity with A. hydrophilaand E. coliindicating their importance in processing of rRNA molecules. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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