| Autor: |
Nayak, Ramesh K., Wu, Arthur S. H. |
| Zdroj: |
Journal of Animal Science; October 1975, Vol. 41 Issue: 4 p1077-1083, 7p |
| Abstrakt: |
The distribution of adenosine triphosphatase (ATP-ase) in prepubertal rabbit, intact and ovariectomized, oviductal epithelium under different hormonal influences was studied by examining the cytochemical products in the cell with an electron microscope. ATP-ase activity was found: (a) on the cell membrane, (b) on the membrane of cilia and microvilli, (c) in the Golgi cisternae and (d) in the basal cell membrane and basement membrane. Mitochondrial ATP-ase was also demonstrated by this procedure. The intensity of the enzyme reaction product at both the intracellular and extracellular sites was significantly reduced following oophorectomy and became distinctly more pronounced following the treatment with estrogen or progesterone. The effects of these two hormones appeared to be synergistic. Estrogen appeared to exert a significant effect on the ATP-ase activity of oviductal cilia.Acid phosphatase activity was also examined. It occurred within the Golgi cisternae, few secretory granules, cell membrane and microvilli of rabbit oviductal epithelium. The enzyme activity was also present within the large membrane-bound bodies similar to the dense bodies described in other cell types. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
