Alkaloids from Peumus boldusand their Acetylcholinesterase, Butyrylcholinesterase and Prolyl Oligopeptidase Inhibition Activity

Autor: Hošt'álková, Anna, Opletal, Lubomír, Kuneš, Jiří, Novák, Zdeněk, Hrabinová, Martina, Chlebek, Jakub, Čegan, Lukáš, Cahlíková, Lucie
Zdroj: Natural Product Communications; April 2015, Vol. 10 Issue: 4
Abstrakt: Eleven isoquinoline alkaloids (1–11) were isolated from dried leaves of Peumus boldusMol. by standard chromatographic methods. The chemical structures were elucidated by MS, and 1D and 2D NMR spectroscopic analysis, and by comparison with literature data. Compounds isolated in sufficient amount were evaluated for their acetylcholinesterase, and butyrylcholinesterase inhibition activity using Ellman's method. In the prolyl oligopeptidase assay, Z-Gly-Pro-p-nitroanilide was used as substrate. Promising butyrylcholinesterase inhibition activities were demonstrated by two benzylisoquinoline alkaloids, reticuline (8) and N-methylcoclaurine (9), with IC50values of 33.6 ± 3.0 μM and 15.0 ± 1.4 μM, respectively. Important prolyl oligopeptidase inhibition activities were shown by N-methyllaurotetanine (6) and sinoacutine (4) with IC50values of 135.4 ± 23.2 μM and 143.1 ± 25.4 μM, respectively. Other tested compounds were considered inactive.
Databáze: Supplemental Index